Having completed the Protein tethering kits (cysteine or ybbR), we can keep supplying you with DNA handles and beads so that you can keep running experiments. This kit is a recurring product that provides you with materials for at least two weeks of continuous measurements.
This kit requires the previous completion of one of the following:
- Biotin- and digoxigenin-modified DNA handles (520 bp per handle)
- Streptavidin-coated polystyrene beads (1.76 µm)
- Anti-digoxigenin coated polystyrene beads (0.75 µm)
- Estimated for 2 weeks of measurement
- Estimated 1–3 weeks delivery time
Biotin-modified and digoxigenin- modified handles
One tube containing an amount of handles mix sufficient for 10 labeling reactions. Aliquot, snap freeze, and store at -80°C.
Polystyrene streptavidin beads (1.76 µm)
25 µl – 1% (w/v) (typical dilution factor 1:1000) streptavidin coated polystyrene microspheres with a diameter of 1.76 µm. Binds to biotin-modified DNA handles. Store at 4°C.
Polystyrene anti-digoxigenin beads (0.75 µm)
250 µl – 0.1% (w/v) (typical dilution factor 1:100) anti-digoxigenin coated polystyrene microspheres with a diameter of 0.75 µm. Binds digoxigenin-modified handles. Store at 4°C.
Want to learn more?
See our Protein Folding and Conformational Changes application notes.
Want to use this product in practice?
Find out more about the C-Trap here.