Easier, more reproducible data – starting with the LUMICKS protocols
The single-stranded DNA is shipped as double-stranded DNA that is biotinylated at the 5’ and 5’ ends of the same strand. After melting the DNA one of the strands will fall off and a single stand remains.
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The Protein labeling and tethering kit (ybbR) is a highly efficient kit to study protein dynamics at a single-molecule level through optical tweezers. The methodology is divided into two main procedures: 1. Protein labeling with functionalized DNA handles, 2. Protein tethering to beads for optical tweezers experiments.
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The single-stranded DNA is shipped as double-stranded DNA that is biotinylated at the 5’ and 5’ ends of the same strand. After melting the DNA one of the strands will fall off and a single stand remains.
Click the button below to download the protocol!
When using any of the standard reusable LUMICKS flow cells, it is important to follow robust cleaning protocols between experiments to avoid cross-contamination.
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DNA structural mechanics kit (for training) protocol
Fluorescent intercalators can bind between basepairs and are very suitable to visualize double-stranded DNA.
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The length of the nick-free biotinylated double-stranded λ-DNA (48,502 bp) makes it ideal for assessing different types of DNA binding molecules. Exposing the DNA to fluorescently labeled proteins enables you to see dynamic binding activities and quantify DNA-associated events, such as replication, repair, organization, or transcription.
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The Protein labeling and tethering kit (Cysteine) is a highly efficient system to study protein dynamics at a single-molecule level through optical tweezers. The methodology is divided into two main procedures: 1. Protein labeling with functionalized DNA handles, 2. Protein tethering to beads for optical tweezers experiments.
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T7 DNA polymerase will either include (polymerization) or exclude (exonucleolysis) basepairs, depending on the force that you apply. This kit allows you to study these kinetics of enzymatic activities associated with DNA replication.
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