Easier, more reproducible data – starting with the LUMICKS protocols

Protocol

Generating single-stranded DNA

The single-stranded DNA is shipped as double-stranded DNA that is biotinylated at the 5’ and 3’ ends of the same strand. Click to learn more.

The single-stranded DNA is shipped as double-stranded DNA that is biotinylated at the 5’ and 3’ ends of the same strand. After melting the DNA one of the strands will fall off and a single stand remains.

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Protocol

Protein labeling and tethering kit (ybbR)

The Protein labeling and tethering kit (ybbR) is a highly efficient kit to study protein dynamics at a single-molecule level through optical tweezers.

The Protein labeling and tethering kit (ybbR) is a highly efficient kit to study protein dynamics at a single-molecule level through optical tweezers. The methodology is divided into two main procedures: 1. Protein labeling with functionalized DNA handles, 2. Protein tethering to beads for optical tweezers experiments.

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Protocol

u-Flux flow cell cleaning protocol

When using any of the standard reusable LUMICKS flow cells, it is important to follow robust cleaning protocols between experiments to avoid cross-contamination. Click here to learn more.

When using any of the standard reusable LUMICKS flow cells, it is important to follow robust cleaning protocols between experiments to avoid cross-contamination.

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Protocol

DNA structural mechanics kit (for training)

DNA structural mechanics kit (for training) protocol

Fluorescent intercalators can bind between basepairs and are very suitable to visualize double-stranded DNA. Click here to learn more.

DNA structural mechanics kit (for training) protocol

Fluorescent intercalators can bind between basepairs and are very suitable to visualize double-stranded DNA.

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Protocol

Protein labeling and tethering kit (cysteine)

The Protein labeling and tethering kit (Cysteine) is a highly efficient system to study protein dynamics at a single-molecule level through optical tweezers. Click here to learn more.

The Protein labeling and tethering kit (Cysteine) is a highly efficient system to study protein dynamics at a single-molecule level through optical tweezers. The methodology is divided into two main procedures: 1. Protein labeling with functionalized DNA handles, 2. Protein tethering to beads for optical tweezers experiments.

Click the button below to download the protocol!

Protocol

DNA–protein interactions kit (for training)

T7 DNA polymerase will either include (polymerization) or exclude (exonucleolysis) basepairs, depending on the force that you apply. Click here to learn more.

T7 DNA polymerase will either include (polymerization) or exclude (exonucleolysis) basepairs, depending on the force that you apply. This kit allows you to study these kinetics of enzymatic activities associated with DNA replication.

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