Easier, more reproducible data – starting with the LUMICKS protocols

Protocol

Biotinylated double-stranded/single-stranded DNA hybrid (17,853 nt)

This DNA construct is ideal for studying protein binding to single-stranded DNA and enzymatic activity such as DNA polymerization, proofreading and replication pause states.

Download
Protocol

DNA tethering kit

The DNA tethering kit is a highly efficient system to tether a DNA sequence of interest (SOI) in optical tweezers and study dynamics of DNA-binding proteins.

Download
Protocol

Nucleosome tethering kit

Using the DNA tethering kit, nucleosomes can be tethered in optical tweezers to study how DNA binding proteins and nucleosomes interact and regulate each other.

Download
Protocol

Generating single-stranded DNA

The single-stranded DNA is shipped as double-stranded DNA that is biotinylated at the 5’ and 5’ ends of the same strand. After melting the DNA one of the strands will fall off and a single stand remains.

Download
Protocol

Protein labeling and tethering kit (cysteine)

The Protein labeling and tethering kit (Cysteine) is a highly efficient system to study protein dynamics at a single-molecule level through optical tweezers.

Download
Protocol

u-Flux flow cell cleaning and passivation protocol

When using any of the standard reusable LUMICKS flow cells, it is important to follow robust cleaning protocols between experiments to avoid cross-contamination. The protocol also describes a passivation protocol for passivating channels used for DNA-binding protein experiments.

Download
Protocol

Protein labeling and tethering kit (ybbR)

The Protein labeling and tethering kit (ybbR) is a highly efficient kit to study protein dynamics at a single-molecule level through optical tweezers.

Download
Protocol

DNA structural mechanics kit (for training)

Fluorescent intercalators can bind between basepairs and are very suitable to visualize double-stranded DNA.

Download
Protocol

Hairpin labeling and tethering kit (long handles)

The hairpin tethering kit is a highly efficient system to characterize secondary DNA/RNA structures using high-resolution single-molecule optical tweezers.

Download
Protocol

Protein labeling and tethering kit (ybbR)

The Protein labeling and tethering kit (ybbR) is a highly efficient kit to study protein dynamics at a single-molecule level through optical tweezers.

Download
Protocol

Biotinylated double-stranded DNA (48,502 bp)

The length of the biotinylated double-stranded λ-DNA (48,502 bp) makes it ideal for assessing different types of DNA binding molecules.

Download
Protocol

Purification of 6xHis AdK oligo

The purification of 6xHis AdK-oligo uses His SpinTrapᵀᴹ (GE28-4013-53).

Download
Protocol

Protocols for oxygen scavenging systems

To prevent damage to (short) DNA tethers or bleaching of fluorophores by reactive oxygen species, it is recommended to use an oxygen scavenging system

Download
Protocol

Hairpin labeling and tethering kit (short handles)

The hairpin tethering kit is a highly efficient system to characterize secondary DNA/RNA structures using high-resolution single-molecule optical tweezers.

Download