This DNA construct is ideal for studying protein binding to single-stranded DNA and enzymatic activity such as DNA polymerization, proofreading and replication pause states.
The DNA tethering kit is a highly efficient system to tether a DNA sequence of interest (SOI) in optical tweezers and study dynamics of DNA-binding proteins.
Using the DNA tethering kit, nucleosomes can be tethered in optical tweezers to study how DNA binding proteins and nucleosomes interact and regulate each other.
The single-stranded DNA is shipped as double-stranded DNA that is biotinylated at the 5’ and 5’ ends of the same strand. After melting the DNA one of the strands will fall off and a single stand remains.
The Protein labeling and tethering kit (Cysteine) is a highly efficient system to study protein dynamics at a single-molecule level through optical tweezers.
When using any of the standard reusable LUMICKS flow cells, it is important to follow robust cleaning protocols between experiments to avoid cross-contamination. The protocol also describes a passivation protocol for passivating channels used for DNA-binding protein experiments.
The Protein labeling and tethering kit (ybbR) is a highly efficient kit to study protein dynamics at a single-molecule level through optical tweezers.
Fluorescent intercalators can bind between basepairs and are very suitable to visualize double-stranded DNA.
The hairpin tethering kit is a highly efficient system to characterize secondary DNA/RNA structures using high-resolution single-molecule optical tweezers.
The length of the biotinylated double-stranded λ-DNA (48,502 bp) makes it ideal for assessing different types of DNA binding molecules.
The purification of 6xHis AdK-oligo uses His SpinTrapᵀᴹ (GE28-4013-53).
The hairpin tethering kit is a highly efficient system to characterize secondary DNA/RNA structures using high-resolution single-molecule optical tweezers.
To prevent damage to (short) DNA tethers or bleaching of fluorophores by reactive oxygen species, it is recommended to use an oxygen scavenging system
The length of the biotinylated and digoxigenin-labeled double-stranded λ-DNA (48,524 bp) makes it ideal for assessing different types of DNA binding molecules. Explore the protocol to find out more.
