DNA handles for Protein tethering kits

$510

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SKU: 00007 Category:

This package provides DNA handles and beads for 10 additional experimental sessions on the C-Trap using oligonucleotide-labeled proteins obtained with the protein labeling and tethering kits. 

For the DNA handles, two choices are available. The short handles (529 bp) will result in the highest force resolution, while the long handles (1506 bp) allow a more straightforward tethering procedure.

Attention!

This kit requires the previous completion of one of the following:

Or can be complemented by one of the following: 


Estimated for 10 experimental sessions on the C-Trap

Estimated 1–4 weeks delivery time

Specifications

Biotinylated and digoxigenin-labeled DNA handles (529 bp or 1506 bp):

45 μl, 1 vial. Handles mix (50/50) with an overhang complementing the maleimide-modified or CoA-modified oligonucleotides. Store at -80°c

Streptavidin-coated polystyrene beads (⌀ 1.0–1.4 μm):

12 μl | 1% (w/v). Beads in PBS with 3 mM sodium azide, with a specific diameter (e.g., 1.2 μm) within the given range. Store at +4°C

Anti-digoxigenin-coated polystyrene beads (⌀ 0.7–0.9 μm):

60 μl | 0.1% (w/v). Beads in PBS with 3 mM sodium azide, with a specific diameter (e.g., 0.8 μm) within the given range. Store at +4°C

Product Resources

Want to learn more?

See our Protein Folding and Conformational Changes application notes.

Want to use this product in practice?

Find out more about the C-Trap here.

Download the Full Specifications or View the Experimental Protocols

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