DNA handles for Protein tethering kits (recurring)

$536

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Having completed the Protein tethering kits (cysteine or ybbR), we can keep supplying you with DNA handles and beads so that you can keep running experiments. This kit is a recurring product that provides you with materials for at least two weeks of continuous measurements.

Attention!

This kit requires the previous completion of one of the following:

Estimated for 2 weeks of measurement

Estimated 1–3 weeks delivery time

Specifications

Biotinylated and digoxigenin-labeled DNA handles (529 bp):

4 μl, 10 vials. Handles mix (50/50) with an overhang complementing the maleimide-modified or CoA-modified oligonucleotides. Store at -80°c

Streptavidin-coated silica beads (⌀ 1.0–1.4 μm):

25 μl | 1% (w/v). Beads in PBS with 3 mM sodium azide, with a specific diameter (e.g., 1.2 μm) within the given range. Store at +4°C

Anti-digoxigenin-coated polystyrene beads (⌀ 0.7–0.9 μm):

60 μl | 0.1% (w/v). Beads in PBS with 3 mM sodium azide, with a specific diameter (e.g., 0.8 μm) within the given range. Store at +4°C

Product Resources

Want to learn more?

See our Protein Folding and Conformational Changes application notes.

Want to use this product in practice?

Find out more about the C-Trap here.

Download the Full Specifications or View the Experimental Protocols