* Update — We have added an alkaline cleaning reagent that is faster and more effective for bead, protein, and lipid removal than the previously provided cleaning reagent. When using intercalating dyes, we suggest using the original cleaning reagent. The provided default passivation buffers (BSA + Pluronics) have also been replaced by Casein, a single step passivation buffer. It is still possible to select BSA + Pluronics as an option. —
The Buffer solutions and beads ‘Basics’ kit includes all the standard materials for your optical tweezers experiments. The materials are estimated for 3 months of C-Trap use.
The ‘Basics’ kit contains the following:
- Phosphate-buffered saline (PBS) based running buffer 10x
- Cleaning reagents*
- Passivation buffer: choose between casein (recommended) or BSA + Pluronics*
- Streptavidin or Anti-digoxigenin coated beads of different sizes
The kit contains polystyrene beads to attain the best trapping conditions depending on your scientific queries and experimental designs. While large diameter beads are easier to trap and are suitable for long DNA tethers (> 3000 bp), small diameter beads are particularly advantageous for short DNA tethers (< 3000 bp) and to measure protein unfolding and conformational changes at the nanoscale.
Estimated 1-4 weeks delivery time.