Biotinylated DNA (48,502 bp) with U:A mismatch and ATTO 647N

$670

SKU: 00022 Categories: ,

This biotinylated double-stranded λ-DNA (48,502 bp) is modified with a U:A mismatch at location 33,786 bp and an ATTO 647N fluorophore at location 44,826 bp. The lesion allows studying DNA mismatch repair pathways such as base excision DNA repair. The fluorophore facilitates determination of the tether directionality, localization of the imaged protein in the DNA sequence and can be used to bring the sample into focus. For visualizing the fluorophore, the 638/639 nm laser of the C-Trap is required.

The DNA is labeled with 3 biotins on each 3’ end to enable tethering in optical tweezers. The DNA lesion will be present in 90% of the tethers containing the fluorophore. Typically, 70% of the tethers contain both the lesion and the fluorophore. 

The other available lesions are G:T mismatch, U:G mismatch and O6 Methyl dG. For request on other DNA lesions, please contact store@lumicks.com.

For the tethering procedure have a look at the Biotinylated double-stranded DNA (48,502 bp) protocol and for the recommended buffers, have a look at the Protocols for oxygen scavenging systems.

 

 

Estimated for 20 experimental sessions on the C-Trap.

Estimated 1-3 weeks delivery time.

Specifications

Biotinylated double-stranded DNA (48,502 bp) with U:A mismatch and ATTO 647N:

20 μl | 20 ng/μl (typical dilution factor 1:1000). Bacteriophage λ-DNA in TE buffer (10 mM Tris HCl pH 8.0 and 1 mM EDTA). Store at +4°C.

Product resources

Want to learn more?

See our DNA/RNA–Protein Interactions application notes.

Want to use this product in practice?

Find out more about the C-Trap here.

Download the Genomic Sequence