Biotinylated DNA (48,502 bp) with 2x ATTO 647N

$595

SKU: 00020 Category:

This biotinylated double-stranded λ-DNA (48,502 bp) is labeled with two ATTO 647N fluorophores, one at position 33,786 bp and one at position 44,836 bp. This will facilitate fluorescence imaging and precise localization of the imaged proteins in the DNA sequence. The DNA is labeled with 3 biotins on each 3’ end to enable tethering in optical tweezers. The spacing between the two fluorophores can be used as a ruler to precisely localize the imaged proteins in the DNA sequence. Typically, 70% of the tethers contain both fluorophores. For visualizing the fluorophores, the 638/639 nm laser of the C-Trap is required. 

To study DNA-repair using optical tweezers, also have a look at the DNA lesion kits.

For the tethering procedure have a look at the Biotinylated double-stranded DNA (48,502 bp) protocol and for the recommended buffers, have a look at the Protocols for oxygen scavenging systems.

Estimated for 20 experimental sessions on the C-Trap.

Estimated 1-3 weeks delivery time.

Specifications

Biotinylated double-stranded DNA (48,502 bp) with 2x ATTO 647N:

20 μl | 20 ng/μl (typical dilution factor 1:1000). Bacteriophage λ-DNA in TE buffer (10 mM Tris HCl pH 8.0 and 1 mM EDTA). Store at +4°C.