The DNA structural mechanics kit (for training) is the standard kit for your first optical tweezers experiments. This kit is perfect for calibrating your optical- tweezers equipment and training new users. You can use fluorescent intercalators (SYTOX Orange, YOYO-1, or DiTO-3) to visualize force-dependent structural changes on a DNA molecule (for more information on biotinylated double-stranded DNA with 2xATTO 647N included in the kit, see this page. Kits ordered before July 2023 contain DNA without ATTO647N fluorophores). Their specificity to double-stranded DNA enables to distinguish these regions from single-stranded DNA regions that are formed upon DNA denaturation induced by stretching the molecule.
A schematic illustration depicting a double-stranded DNA tethered between two optically trapped beads. Force-mediated stretching of the DNA molecule contributes to the formation of single-stranded DNA. Read more about the application here.
Estimated for 10 experimental sessions on the C-Trap.
Estimated 1–4 weeks delivery time