DNA structural mechanics kit (for training)

$810

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SKU: 00012 Category:

The DNA structural mechanics kit (for training) is the standard kit for your first optical tweezers experiments. This kit is perfect for calibrating your optical- tweezers equipment and training new users. You can use fluorescent intercalators (SYTOX Orange, YOYO-1, or DiTO-3) to visualize force-dependent structural changes on a DNA molecule (for more information on biotinylated double-stranded DNA included in the kit, see this page). Their specificity to double-stranded DNA enables to distinguish these regions from single-stranded DNA regions that are formed upon DNA denaturation induced by stretching the molecule.

A schematic illustration depicting a double-stranded DNA tethered between two optically trapped beads. Force-mediated stretching of the DNA molecule contributes to the formation of single-stranded DNA. Read more about the application here. 

Estimated for 10 experimental sessions on the C-Trap. 

Estimated 1–4 weeks delivery time

Specifications

Biotinylated double-stranded DNA (48,502 bp):

20 μl | 20 ng/μl. Bacteriophage λ-DNA in TE buffer (10 mM Tris HCl pH 8.0 and 1 mM EDTA). Store at +4°C

SYTOX Orange / YOYO-1 / DiTO-3:

5 μl | 5 mM / 1 mM / 5 mM. Choose between SYTOX Orange (preferred option, green laser required), YOYO-1 (blue laser required), and DiTO-3 (choose when only red laser present). Store at -20°C

Streptavidin-coated polystyrene beads (⌀ 4.0–4.9 μm):

200 μl | 0.5% (w/v). Beads in PBS with 3 mM sodium azide, with a specific diameter (e.g., 4.5 μm) within the given range. Store at +4°C

Product Resources

Want to learn more?

Download the protocol.

See our DNA/RNA–Protein Interactions application notes.

Want to use this product in practice?

Find out more about the C-Trap here.

 

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