The length of the biotinylated and digoxigenin-labeled double-stranded λ-DNA (48,524 bp) makes it ideal for assessing different types of DNA binding molecules. The differently labeled ends of the DNA (3 digoxigenins on the 3’ end and 5 biotins on the 5’ end of the same strand) enables to determine the directionality of λ-DNA trapped in optical tweezers. . Exposing the DNA to fluorescently labeled proteins enables you to monitor dynamic binding activities and quantify DNA-associated events, such as replication, repair, organization, or transcription. The double-stranded DNA can also be visualized by fluorescent intercalators. For more information, have a look at the DNA structural mechanics kit.
Estimated for 20 experimental sessions on the C-Trap.
Estimated 1–3 weeks delivery time