DNA structural mechanics kit

$654

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The DNA structural mechanics kit is the standard kit for your first optical tweezers experiments. This kit is perfect for calibrating your optical-tweezers equipment and training new users. You can use fluorescent intercalators (for example, SYTOX Orange, YOYO-1, or Sytox Red) to visualize force-dependent structural changes on a DNA molecule. Their specificity to double-stranded DNA enables you to distinguish these regions from single-stranded DNA regions that are formed upon  DNA denaturation induced by stretching the molecule.

A schematic illustration depicting a double-stranded DNA tethered between two optically trapped beads. Force-mediated stretching of the DNA molecule contributes to the formation of single-stranded DNA. Read more about the application here

Material supplied:

  • Biotinylated double-stranded λ-DNA
  • Fluorescent intercalator, choose between SYTOX Orange (preferred option, green laser required), YOYO-1 (blue laser required), and SYTOX Red (choose when only red laser present)
  • Streptavidin-coated polystyrene beads (4.5 µm)
  • Estimated for 2 weeks of measurements
  • Estimated 1–3 weeks delivery time

 

Specifications

Biotinylated double-stranded DNA (48,502 bp):

20 μl | 20 ng/μl. Bacteriophage λ-DNA in TE buffer (10 mM Tris HCl pH 8.0 and 1 mM EDTA). Store at +4°C

SYTOX Orange / YOYO-1 / DiTO-3:

5 μl. Dye in DMSO. Store at -20°C

Streptavidin-coated polystyrene beads (⌀ 4.0–4.9 μm):

600 μl | 0.5% (w/v). Beads in PBS with 3 mM sodium azide, with a specific diameter (e.g., 4.5 μm) within the given range. Store at +4°C

Download the Full Specifications or View the Experimental Protocols

Product Resources

Want to learn more?

See our DNA/RNA–Protein Interactions application notes.

Want to use this product in practice?

Find out more about the C-Trap here.