Hairpin labeling and tethering kit

$730

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SKU: 00016/17 Category:

The hairpin labeling and tethering kit provides a highly efficient system to characterize DNA/RNA structures using high-resolution single-molecule optical tweezers. Short handles will result in a higher force resolution, while long handles are better suitable for experimental workflows involving fluorescence. 

The labeling procedure relies on a DNA/RNA hairpin ligation to the functionalized DNA handles. The hairpin of interest (provided by the user) requires a flanking 15 nt DNA sequence both at the 5’ end and the 3’ end.

The kit contains a control hairpin containing the flanking 15 nt DNA sequences that can be used as quality control for the labeling and tethering procedures. For the sequence of the control hairpin, have a look at the tab “Product resources”. 

Our hairpin labeling and tethering kit relieves you from costs and time that are typically required for personnel training, troubleshooting, experiment optimization, and custom application development.


The kit enables 10 hairpin labelings 

Estimated 1-4 weeks delivery time

Specifications

  • Biotinylated (1045 bp or 2512 bp) and digoxigenin-labeled (1052 bp or 2528 bp) DNA handles:

85 μl. Handles mix (50/50) with overhangs that anneal with the hairpin’s flanking sequences. Store at -20 °C.

  • DNA Ligase:

10 μl. Store at -20 °C.

  • 10x Ligase buffer: 

25 μl | 10x. Store at -20 °C.

  • Control DNA (hairpin)

10 μl | 5 μM. DNA hairpin that can anneal with the handles’ overhangs that can be used as a quality control. Store at -20 °C.

  • Streptavidin coated polystyrene beads (⌀ 1.0-1.4 µm): 

12 μl | 1% (w/v). Beads in PBS with 3 mM sodium azide, with a specific diameter (e.g 1.36 μm) within the given range. Store at +4 °C.

  • Anti-digoxigenin-coated polystyrene beads (⌀ 0.7-0.9 µm):

60 μl | 0.1% (w/v). Beads in PBS with 3 mM sodium azide, with a specific diameter (e.g. 0.86 μm) within the given range. Store at +4 °C.

Product Resources

Want to learn more?

Download the protocolS:

See our DNA/RNA–Protein Interactions application notes.

Want to use this product in practice?

Find out more about the C-Trap here.

Download the Genome Sequence:

  1. Biotinylated DNA handle (1045 bp).dna
  2. Biotinylated DNA handle (2512 bp).dna
  3. Control DNA (hairpin).dna
  4. Digoxigenin-labeled DNA handle (1052 bp).dna
  5. Digoxigenin-labeled DNA handle (2528 bp).dna

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