Beads & Cleaning kit – Recommended

€750 – €830

SKU: 11228 Category:

The Beads & Cleaning Kit – Recommended includes the Alkaline Cleaning Reagent (ACR), designed to thoroughly clean the microfluidics module of the C-Trap. ACR is highly efficient at eliminating biological materials such as DNA, proteins, lipids, and bioconjugated polystyrene beads. Its powerful cleaning action breaks down and lifts contaminants, leaving the microfluidics module clean and ready for reliable performance in sensitive single-molecule experiments.

Thanks to its superior performance compared to bleach-based cleaning reagents, ACR enables the use of high concentrations of passivating agents, such as 2% bovine serum albumin (BSA) and 1% casein. Although the kit does not include passivation materials, we provide recommendations for suitable products and their preparation.

In addition to ACR, the kit contains two tubes of polystyrene beads, selectable via our webstore based on bead diameter and surface coating (Streptavidin or Anti-Digoxigenin) to match your experimental needs.

The included ACR and beads provide sufficient material for up to 60 cleaning cycles and 60 experimental sessions in the C-Trap.

For workflows involving DNA intercalating dyes, we offer two optional reagents: the Cleaning Reagent (1x) and the Reducing Reagent (30x).
Important: The Cleaning Reagent (1x) is different from ACR and should be used only for the removal of intercalating dyes (see protocols in Product Resources)

As an additional add-on, microfluidics syringes (pack of 100) are available to support routine replacement after each cleaning cycle.

Cleaning and passivation performance can be evaluated using the C-Trap Reference Kit, which includes a standardized DNA–protein interaction assay to assess microfluidics cleanliness, tether formation, and optics alignment.

Specifications

Store at Room Temperature:

  • Alkaline cleaning reagent: 12 ml | 50x concentration.
    Hazardous material. Contains tripotassium phosphate and potassium hydroxide.

  • (Optional) Cleaning reagent: 300 ml | 1x concentration.
    Hazardous material. Contains sodium hypochlorite and sodium hydroxide.

  • (Optional) Reducing agent: 15 ml | 30x concentration.
    Contains sodium thiosulphate.

  • (Optional) Syringes: 100 pieces.

Bead Selection – Store at 4°C:

  • Streptavidin-coated beads (⌀ 1.0–1.4 µm): 60 µl | 1.0% (w/v).
    Dilution factor: 1:1000.

  • Streptavidin-coated beads (⌀ 1.5–1.9 µm): 60 µl | 1.0% (w/v).
    Dilution factor: 1:1000.

  • Streptavidin-coated beads (⌀ 4.0–4.9 µm): 600 µl | 0.5% (w/v).
    Dilution factor: 1:100.

  • Anti-digoxigenin-coated beads (⌀ 0.7–0.9 µm): 600 µl | 0.1% (w/v).
    Dilution factor: 1:100.
  • Anti-digoxigenin-coated beads (⌀ 2.0–2.4 µm): 600 µl | 0.1% (w/v).
    Dilution factor: 1:100.

All beads are polystyrene and supplied in PBS with 3 mM sodium azide.
Exact diameter is indicated on the tube label.

Product resources

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