Easier, more reproducible data – starting with the LUMICKS protocols

Protocol

C-Trap Reference Kit Protocol

The C-Trap Reference Kit is designed to support user training, fast-track assay troubleshooting, and verify instrument performance. The kit provides standardized and validated reagents for a complete DNA-protein binding
assay, including data analysis.

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Protocol

C-Trap Reference Kit Quick Protocol

For a fast-reference version, download the quick protocol of the C-Trap Reference Kit.

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Protocol

C-Trap Cleaning and Passivation Protocol – Recommended

The cleaning and passivation procedures described in this protocol rely on the materials provided by the Beads & Cleaning Kit – Recommended.

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Protocol

C-Trap Cleaning and Passivation Quick Protocol – Recommended

For a fast-reference version, download the quick protocol of the C-Trap Cleaning and Passivation Protocol – Recommended.

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Protocol

Hairpin labeling and tethering kit (short handles)

The hairpin tethering kit is a highly efficient system to characterize secondary DNA/RNA structures using high-resolution single-molecule optical tweezers.

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Protocol

DNA tethering kit

The DNA tethering kit is a highly efficient system to tether a DNA sequence of interest (SOI) in optical tweezers and study dynamics of DNA-binding proteins.

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Protocol

Cleaning and Passivation Protocol – Legacy

The cleaning and passivation procedures described in this protocol rely on the materials provided by the Beads & Cleaning Kit – Legacy.

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Protocol

DNA Repeat assembly

Assembling biotinylated DNA repeat array with up to 50 copies of your DNA sequence of interest allowing high-throughput data acquisition in the C-Trap.

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Protocol

Hairpin labeling and tethering kit (long handles)

The hairpin tethering kit is a highly efficient system to characterize secondary DNA/RNA structures using high-resolution single-molecule optical tweezers.

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Protocol

Creating DNA constructs with multiple custom sequences using the Golden Gate method

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Protocol

Biotinylated Lambda DNA

The length of the biotinylated double-stranded λ-DNA (48,502 bp) makes it ideal for assessing different types of DNA binding molecules.

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Protocol

Biotinylated DNA Hybrid

This DNA construct is ideal for studying protein binding to single-stranded DNA and enzymatic activity such as DNA polymerization, proofreading and replication pause states.

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Protocol

Generating single-stranded DNA

The single-stranded DNA is shipped as double-stranded DNA that is biotinylated at the 5’ and 5’ ends of the same strand. After melting the DNA one of the strands will fall off and a single stand remains.

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Protocol

Nucleosome tethering kit

The nucleosome tethering kit enables the tethering of nucleosomal arrays in optical tweezers to study the interactions and regulation between DNA binding proteins and nucleosomes.

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Protocol

Nucleosome microscale assembly protocol

Using the Nucleosome tethering kit, nucleosomes can be tethered in optical tweezers to study how DNA binding proteins and nucleosomes interact and regulate each other.

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Protocol

DNA structural mechanics kit (for training)

Fluorescent intercalators can bind between basepairs and are very suitable to visualize double-stranded DNA.

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Protocol

Biotin Digoxigenin Lambda DNA

The length of the biotinylated and digoxigenin-labeled double-stranded λ-DNA (48,524 bp) makes it ideal for assessing different types of DNA binding molecules. Explore the protocol to find out more.

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Protocol

Purification of 6xHis AdK-oligo

The purification of 6xHis AdK-oligo uses His SpinTrapᵀᴹ (GE28-4013-53).

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Protocol

Protein Labeling and Tethering Kit (ybbR)

The Protein labeling and tethering kit (ybbR) enables the labeling of your protein of interest with DNA handles suitable for tethering in the C-Trap

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Protocol

Protein Labeling and Tethering Kit (cysteine)

The Protein labeling and tethering kit (Cysteine) enables the labeling of your protein of interest with DNA handles suitable for tethering in the C-Trap

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Quick Protocol

Protein Labeling and Tethering Kit (ybbR)

The Protein labeling and tethering kit (ybbR) enables the labeling of your protein of interest with DNA handles suitable for tethering in the C-Trap

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Quick Protocol

Protein Labeling and Tethering Kit (cysteine)

The Protein labeling and tethering kit (Cysteine) enables the labeling of your protein of interest with DNA handles suitable for tethering in the C-Trap

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