The Running Reagents and Beads ‘Basics’ kit includes all the standard materials for your optical tweezers experiments. The materials are estimated for 3 months of system running.
The ‘Basics’ kit contains the following:
- Phosphate-buffered saline (PBS) based running buffer
- Cleaning reagents
- Passivation buffer
- Streptavidin or Anti-digoxigenin coated beads of different sizes
- Fluidics syringes
The kit contains polystyrene beads to attain the best trapping conditions depending on your scientific queries and experimental designs. While large diameter beads are easier to trap and are suitable for long DNA tethers (> 3000 bp), small diameter beads are particularly advantageous for short DNA tethers (< 1500 bp) and to measure protein unfolding and conformational changes at the nanoscale.