Running reagents and beads kit

600

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The Running Reagents and Beads ‘Basics’ kit includes all the standard materials for your optical tweezers experiments. The materials are estimated for 3 months of system running.

The ‘Basics’ kit contains the following:

  • Phosphate-buffered saline (PBS) based running buffer
  • Cleaning reagents
  • Passivation buffer
  • Streptavidin or Anti-digoxigenin coated beads of different sizes
  • Fluidics syringes

The kit contains polystyrene beads to attain the best trapping conditions depending on your scientific queries and experimental designs. While large diameter beads are easier to trap and are suitable for long DNA tethers (> 3000 bp), small diameter beads are particularly advantageous for short DNA tethers (< 1500 bp) and to measure protein unfolding and conformational changes at the nanoscale.

Specifications

Running buffer: 75 ml (typical dilution factor 1:10) Phosphate-saline buffer with 50 mM sodium azide (NaN3) and 5 mM EDTA.

Cleaning reagents: 300 ml 0.7 M NaClO (bleach). 3 ml 0.5 M (typical dilution factor 1:30) Na2S2O3.

Passivation buffer: 15 ml 1% (typical dilution factor 1:10) BSA. 15 ml 5% (typical dilution factor 1:10) Pluronics.

Streptavidin Beads (diameter 0.7–0.9 / 1.5-1.9 µm ): 60 μl (based on single selection of bead size) 1 % (w/v) (typical dilution factor 1:1000) streptavidin-coated polystyrene microspheres. Your batch of beads will have a specific diameter (e.g., 0.82 um) within the 070.9 or 1.5-1.9 μm diameter range selected in the order.

Streptavidin Beads (diameter 4.0–4.9 um): 600 μl (based on single selection of bead size)0.5 % (w/v) (typical dilution factor 1:100) streptavidin-coated polystyrene microspheres. Your batch of beads will have a specific diameter (e.g., 4.5 um) within the 4.04.9 μm diameter range selected in the order.

Anti-Digoxigenin Beads (diameter 0.7–0.9 / 2.0-2.4 µm ): 600 μl (based on single selection of bead size) 0.1 % (w/v) (typical dilution factor 1:100) anti-digoxigenin-coated polystyrene microspheres. Your batch of beads will have a specific diameter (e.g., 0.82 um) within the 070.9 or 2.0-2.4 μm diameter range selected in the order.

Syringes: 50 pieces – 3 ml syringes that perfectly fit in the microfluidics system.

Product Resources

Want to use this product in practice?

Find out more about the C-Trap here.