Running reagents and beads kit

$654

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The Running Reagents and Beads ‘Basics’ kit includes all the standard materials for your optical tweezers experiments. The materials are estimated for 3 months of system running.

The ‘Basics’ kit contains the following:

  • Phosphate-buffered saline (PBS) based running buffer
  • Cleaning reagents
  • Passivation buffer
  • Streptavidin or Anti-digoxigenin coated beads of different sizes
  • Fluidics syringes

The kit contains polystyrene beads to attain the best trapping conditions depending on your scientific queries and experimental designs. While large diameter beads are easier to trap and are suitable for long DNA tethers (> 3000 bp), small diameter beads are particularly advantageous for short DNA tethers (< 1500 bp) and to measure protein unfolding and conformational changes at the nanoscale.

Specifications

Running buffer:

30 ml, 3 containers | (typical dilution factor 1:10). PBS with 50 mM sodium azide and 5 mM EDTA. Store at RT.

Cleaning reagent:

300 ml | 1x. Contains bleach. Store at RT.

Reducing reagent:

15 ml | (typical dilution factor 1:30). Contains sodium thiosulfate. Store at RT.

Passivation buffer BSA:

15 ml | 1%. (Typical dilution factor 1:10). Store at RT.

Passivation buffer Pluronic:

15 ml | 5%. (Typical dilution factor 1:10). Store at RT.

Streptavidin-coated polystyrene beads (⌀ 0.7–0.9 μm / ⌀ 1.5–1.9 μm):

60 μl | 1% (w/v) (typical dilution factor 1:1000). Beads in PBS with 3 mM sodium azide, with a specific diameter (e.g., 0.8 μm) within the given range. Volume based on single selection of bead size. Store at +4°C.

Streptavidin-coated polystyrene beads (⌀ 4.0–4.9 μm):

600 μl | 0.5% (w/v) (typical dilution factor 1:100). Beads in PBS with 3 mM sodium azide, with a specific diameter (e.g., 4.5 μm) within the given range. Volume based on single selection of bead size. Store at +4°C.

Anti-digoxigenin-coated polystyrene beads (⌀ 0.7–0.9 μm / ⌀ 2.0–2.4 μm):

600 μl | 0.1% (w/v) (typical dilution factor 1:100). Beads in PBS with 3 mM sodium azide, with a specific diameter (e.g., 0.8 μm) within the given range. Volume based on single selection of bead size. Store at +4°C.

Download the Full Specifications or View the Experimental Protocols

Product Resources

Want to use this product in practice?

Find out more about the C-Trap here.