The DNA–protein interaction kit can kick-start your single-molecule experiments, allowing you to study the kinetics of enzymatic activities associated with DNA replication. The biotinylated pKYB1 DNA (8,398 bp) contains a 25-nucleotide long 5′-overhang that serves as a loading site for DNA polymerases. This kit is ideal for extracting real-time data of DNA replication processes, including polymerization dynamics, proofreading activities, and pause states.
The DNA–protein interactions kit provides you with a ready-to-go system for single-molecule applications to:
- Obtain millisecond and base-pair resolution data with kinetics and mechanistic information from DNA-replication processes at the single-molecule level.
- Mimic nucleotide miss-incorporation through the application of DNA tension forces, which enables you to measure polymerization rates, exonucleolytic activity, and pause states of DNA polymerase enzymes.
- Test polymerase activity with the u-Flux™ Laminar Flow Microfluidics, which facilitates switching between different experimental conditions in different channels.