DNA–protein interactions kit

$1,199

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The DNA–protein interaction kit can kick-start your single-molecule experiments, allowing you to study the kinetics of enzymatic activities associated with DNA replication. The biotinylated pKYB1 DNA (8,398 bp) contains a 25-nucleotide long 5′-overhang that serves as a loading site for DNA polymerases. This kit is ideal for extracting real-time data of DNA replication processes, including polymerization dynamics, proofreading activities, and pause states.

The DNA–protein interactions kit provides you with a ready-to-go system for single-molecule applications to:

  1. Obtain millisecond and base-pair resolution data with kinetics and mechanistic information from DNA-replication processes at the single-molecule level.
  2. Mimic nucleotide miss-incorporation through the application of DNA tension forces, which enables you to measure polymerization rates, exonucleolytic activity, and pause states of DNA polymerase enzymes.
  3. Test polymerase activity with the u-Flux™ Laminar Flow Microfluidics, which facilitates switching between different experimental conditions in different channels.

Specifications

T7 DNA polymerase:

20 μl, 200 Units | 10,000 U/ml. Polymerase with 8x Histidine tag in 50% glycerol. Store all materials at -20°C.

dNTP mix:

300 μl | 10 mM of each nucleotide.

10x Polymerase buffer:

500 μl | 10x. Store all materials at -20°C.

BSA passivation reagent:

2 ml | 1%. Store all materials at -20°C.

Pluronic passivation reagent:

2 ml | 5%. Store at RT.

Biotinylated double-stranded DNA (8,398 bp) with overhang:

20 μl | 20 ng/μl. Linearized plasmid pKYB1 in TE buffer (10 mM Tris HCl pH 8.0 and 1 mM EDTA). Store all materials at +4°C.

Streptavidin-coated polystyrene beads (⌀ 1.5–1.9 μm):

25 μl | 1% (w/v). Beads in PBS with 3 mM sodium azide, with a specific diameter (e.g., 1.7 μm) within the given range. Store all materials at +4°C.

Download the Full Specifications or View the Experimental Protocols

Product Resources

Want to learn more?

See our DNA/RNA–Protein Interactions application notes.

Want to use this product in practice?

Find out more about the C-Trap here.