C-Trap™ Training & Calibration Kit

C-Trap™ Training & Calibration Kit

900

Product Description

The standard training & calibration kit for your C-Trap™ instrument. This kit is perfect for calibrating the C-Trap™ and training new users, by simultaneously manipulating DNA and visualizing force-dependent binding of fluorescent SYTOX Orange. Contains the following:

  • Biotinylated ds λ-DNA
  • SYTOX Orange
  • Streptavidin coated polystyrene beads (4.5 µm)
  • Immersion oil
  • Phosphate-buffered saline (PBS) based buffer
  • u-Flux™ syringes

Additional Information

ds λ-DNA

50 µl – 20 ng/µl (typical dilution factor 1:1000) double-stranded bacteriophage λ-DNA molecule (48502 bp). Labeled with multiple biotins on the 3′ ends of both strands, diluted in TE buffer (10 nM Tris HCl pH 8.0 and 1 nM EDTA).

SYTOX Orange

50 µl – 500 µM (typical dilution factor 1:10000) SYTOX Orange dye in DMSO. It is optimal to visualize dsDNA because of its force-dependent binding properties on nucleic acids and it only fluoresces when bound to dsDNA.

Polystyrene beads (4.5 µm)

1000 µl – 5% (w/v) (typical dilution factor 1:100) streptavidin coated polystyrene microspheres with a diameter of 4.5 µm in water. Binds to biotinylated DNA (e.g. the provided λ-DNA) and can be optically manipulated using the C-Trap™.

Immersion oil

20 ml – Non-fluorescence immersion oil for condensor immersion.

PBS based buffer

100 ml – Phosphate-buffered saline buffer with 5 mM sodium azide (NaN3) and 0.5 mM EDTA.

u-Flux™ syringes

30 pieces – 3 ml syringes that perfectly fit in the u-Flux™ system.