Biotinylated double-stranded DNA (8,398 bp) with overhang


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This double-stranded pKYB1 DNA (8,398 bp) with a 25-nucleotide long 5′-overhang is biotinylated and nick-free, making it ideal for measuring DNA–protein interactions and enzymatic activities on DNA. The 5’ overhang serves as a loading site for enzymes (for example, T7 DNA polymerase) to study replication processes, including polymerization, proofreading, and replication pause states. The short length of the DNA also makes it ideal for measuring small structural changes of the DNA resulting from, for example, protein binding or buffer conditions.

For more information, have a look at the DNA–protein interactions kit.


  • Estimated for 2 weeks of measurements
  • Estimated 1–3 weeks delivery time


20 µl – 20 ng/µl (typical dilution factor 1:1000) double-stranded expression vector pKYB1 (8393 bp). Biotin-labeled on the 5′ and 3′ ends of one of the strands, diluted in TE buffer (10 mM Tris HCl pH 8.0 and 1 mM EDTA).

Product Resources

Want to learn more?

See our DNA/RNA–Protein Interactions application notes.

Want to use this product in practice?

Find out more about the C-Trap here.