Biotinylated double-stranded DNA (8,398 bp) with overhang

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SKU: 00005 Category:

Attention! This product will be discontinued. For an alternative, please have a look at the biotinylated double-stranded/single-stranded hybrid (17.853 nt).

This double-stranded pKYB1 DNA (8,398 bp) with a 25-nucleotide long 5′-overhang is biotinylated (2 biotins on 3’ end and 3 biotins on 5’ end) and nick-free, making it ideal for measuring DNA–protein interactions and enzymatic activities on DNA. The 5’ overhang serves as a loading site for enzymes (for example, T7 DNA polymerase) to study replication processes, including polymerization, proofreading, and replication pause states. The short length of the DNA also makes it ideal for measuring small structural changes of the DNA resulting from, for example, protein binding or buffer conditions.



Estimated for 20 experimental sessions on the C-Trap

Estimated 1–4 weeks delivery time


Biotinylated double-stranded DNA (8,398 bp) with overhang:

20 μl | 20 ng/μl (typical dilution factor 1:1000). Linearized plasmid pKYB1 with 25 nt overhang in TE buffer (10 mM Tris HCl pH 8.0 and 1 mM EDTA). Store at +4°C.


Product Resources

Want to learn more?

See our DNA/RNA–Protein Interactions application notes.

Want to use this product in practice?

Find out more about the C-Trap here.

Download the Full Specifications or View the Experimental Protocols

Download the Genomic Sequence